CD61 integrin beta 3 monoclonal antibody 2c9g3 from eBioscience is a research tool designed to target the human integrin beta 3 subunit, enabling precise detection and functional studies in immunology and cell adhesion research. This antibody supports flow cytometry, western blot, and immunohistochemistry applications where integrin beta 3 expression and signaling are relevant.
Engineered for high specificity and compatibility with eBioscience workflows, 2c9g3 facilitates the investigation of platelet activation, immune cell migration, and tumor microenvironment interactions mediated by integrin beta 3. Below is a structured overview to help researchers quickly assess its key attributes.
| Product Name | Target | Applications | Format |
|---|---|---|---|
| CD61 integrin beta 3 monoclonal antibody 2c9g3 (eBioscience) | Integrin beta 3 (CD61) | Flow cytometry, Western blot, Immunohistochemistry | Mouse monoclonal, purified IgG |
| Concentration | Formulation | Species Reactivity | Storage |
| Concentration varies by format; refer to specific lot label | Buffer with protease inhibitors | Human | 2–8°C, protected from light |
Integrin Beta 3 Specific Function and Signaling
Integrin beta 3, commonly identified as CD61, is a critical component of several alpha-beta integrin heterodimers, including alphaIIb beta3 and alphaV beta3. The CD61 integrin beta 3 monoclonal antibody 2c9g3 binds specifically to epitopes associated with the activated and resting conformations of beta 3, supporting precise mapping of integrin dynamics in cellular adhesion and signaling studies. Understanding these conformational states is essential for interpreting integrin-mediated pathways in hemostasis, angiogenesis, and immune cell trafficking.
Flow Cytometry and Immunophenotyping Applications
In flow cytometry experiments, the 2c9g3 clone enables reliable detection of integrin beta 3 on platelets, endothelial cells, and selected immune subsets, allowing researchers to monitor changes in receptor expression during activation or differentiation. The antibody’s compatibility with intracellular staining protocols expands its utility in multiplex panels that combine surface and intracellular markers, improving resolution in complex tissue and cell population analyses.
Western Blot and Immunohistochemistry Validation
Validated western blot applications with the CD61 integrin beta 3 monoclonal antibody 2c9g3 provide evidence of specific bands corresponding to the expected molecular weight of integrin beta 3, confirming its presence in lysates from relevant cell lines and tissue samples. In parallel, immunohistochemistry protocols support the visualization of integrin beta 3 in formalin-fixed, paraffin-embedded tissues, helping researchers correlate protein expression with histological context and lesion characteristics.
Advanced Experimental Considerations
When planning experiments with this antibody, evaluating fixation methods, permeabilization steps, and species-specific secondary reagents is essential to achieve reproducible staining and minimal background. Selecting appropriate isotype controls and conducting titration curves further ensures high signal-to-noise ratios across diverse sample types, from cell lines to clinical specimens.
Key Takeaways for Researchers
- Target integrin beta 3 (CD61) with high specificity using the eBioscience 2c9g3 clone.
- Apply the antibody in flow cytometry, western blot, and immunohistochemistry workflows.
- Validate protocols with proper controls and titration for each sample type.
- Consider fixation and permeabilization conditions to preserve epitope accessibility.
- Combine with appropriate secondary reagents and isotype controls for robust data.
FAQ
Reader questions
What biological processes does integrin beta 3 mediate that are relevant to the 2c9g3 antibody?
Integrin beta 3 mediates platelet aggregation, endothelial cell migration, and immune cell adhesion, making the CD61 integrin beta 3 monoclonal antibody 2c9g3 suitable for studies of hemostasis, angiogenesis, and inflammatory responses.
Can this antibody be used for intracellular detection in fixed cells?
Yes, the antibody supports intracellular staining in fixed and permeabilized cell preparations, enabling simultaneous detection of surface and intracellular integrin beta 3 in complex multiplex assays.
How should I determine the optimal working concentration for 2c9g3 in my application?
Perform a titration using positive control cells or tissues, and consult the technical data sheet provided by eBioscience for recommended concentration ranges, isotype controls, and validation details specific to flow cytometry or immunohistochemistry.
What sample preparation steps are critical for reliable results with this antibody?
Optimize fixation and permeabilization conditions, validate blocking buffers to reduce nonspecific binding, and use appropriate secondary reagents matched to the species and isotype of the primary antibody to ensure consistent detection.