Nori Ferret GDF15 ELISA Kit delivers sensitive and specific measurement of Growth Differentiation Factor 15 for stress response and metabolic research. This assay supports exploratory studies in laboratory models and translational projects focused on fibrosis, inflammation, and appetite regulation pathways.
Designed for research use only, the kit provides a streamlined workflow and robust detection performance to help scientists generate reproducible biomarker data efficiently.
| Product Name | Nori Ferret GDF15 ELISA Kit | Target Analyte | Growth Differentiation Factor 15 (GDF15) |
|---|---|---|---|
| Assay Type | Sandwich ELISA | Detection Range | 0.312 to 20 ng/mL |
| Sample Matrix | Serum, Plasma, Cell Culture Supernatant | Incubation Time | 30 min at RT |
| Readout | 450 nm with Stop Solution | Clinical Cohort Fit | Metabolic and cardiovascular studies |
| Species Reactivity | Ferret | Plate Format | 96-well strip plate |
| Standard Curve Range | 0 to 100 ng/mL | Storage | 2–8°C for up to 12 months |
| Limit of Detection | 0.21 ng/mL | Required Equipment | Microplate reader, pipettes, incubator |
| Kit Components | Capture Antibody, Detecting Antibody, Standards, Substrate, Stop Solution | Data Analysis | Standard curve fitting, sample interpolation |
Nor Ferret GDF15 Assay Principle and Workflow
The Nori Ferret GDF15 ELISA Kit employs a solid-phase sandwich format using coated capture antibodies and enzyme-linked detection antibodies. After sample addition and incubation, TMB substrate generates a color signal proportional to GDF15 concentration, which is then measured with a microplate reader.
Each kit contains pre-coated plates, standards, buffers, and enzymes, enabling researchers to set up assays without extensive optimization. The streamlined protocol reduces hands-on time while maintaining sensitivity and precision across biological samples.
Experimental Design and Sample Preparation Tips
Sample Collection and Handling
Collect serum or plasma using anticoagulant tubes suitable for ferret species and centrifuge carefully to remove cellular debris. Avoid hemolysis and store aliquots at −80°C to preserve GDF15 stability before analysis.
Assay Setup and Controls
Include kit standards and quality control samples in each run to verify plate performance and data reliability. Run samples in duplicate or triplicate to minimize variability and ensure accurate quantification across the dynamic range.
Data Analysis and Reporting Parameters
Standard Curve Construction
Plot absorbance versus log concentration to generate a standard curve, then fit a four-parameter logistic model for interpolation. Report GDF15 concentrations in ng/mL alongside raw absorbance values for traceability.
Sensitivity and Performance
Document limit of detection, intra- and inter-assay coefficients of variation, and linearity ranges in method validation records. Align reporting metrics with study objectives to support reproducibility in metabolic and cardiovascular research.
Key Takeaways and Recommendations
- Follow the sample preparation and incubation steps precisely to maintain kit sensitivity.
- Include quality control samples in every run to monitor plate performance and data integrity.
- Validate matrix compatibility and recovery for each new sample type before routine use.
- Store reagents and samples under recommended conditions to maximize assay consistency.
- Document all protocol deviations and performance metrics for regulatory and publication requirements.
FAQ
Reader questions
Can this kit be used for other ferret strains or related species?
No, the antibodies are optimized specifically for ferrets and may not show acceptable cross-reactivity with other species without validation.
How should I handle samples with suspected interfering substances?
Perform matrix effect checks by spiking known GDF15 concentrations into sample matrices and comparing recovery against standards in buffer.
What is the recommended storage condition for prepared standard curves?
Store unused standards and diluent at 2–8°C, and complete plate reading within the recommended timeframe to maintain signal stability.
Is it acceptable to use frozen-thawed samples in this assay?
Yes, freeze-thaw samples carefully and avoid repeated cycles; verify recovery in a pilot study to confirm minimal impact on measured GDF15 levels.