Fluorescein, cataloged as cas 2321075 by AAT Bioquest, is a trusted xanthene dye widely used in biomedical research and diagnostic applications. This concise guide outlines the technical profile, handling protocols, assay compatibility, and storage recommendations for this high-purity reference standard.
Researchers rely on accurately labeled compounds to ensure assay reproducibility and compliance with regulatory expectations. The following specification table and topic sections clarify quality attributes, method integration, and risk mitigation for Fluorescein (cas 2321075) from AAT Bioquest.
| Catalog Item | Fluorescein (cas 2321075) | Purity | Supplier |
|---|---|---|---|
| Catalog Number | AAT-14389 | ≥98% by HPLC | AAT Bioquest |
| Molecular Formula | C20H12O5 | Molecular Weight | 332.31 g/mol |
| Physical Form | Solid powder | Solubility | Water: >10 mg/mL |
| Storage Conditions | 2–8°C, protected from light | Shelf Life | 24 months unopened |
Spectroscopic Characteristics and Detection Compatibility
Fluorescein (cas 2321075) exhibits strong excitation around 494 nm and emission near 512 nm, making it ideal for fluorescence-based readouts. Its absorbance and quantum yield support sensitive detection in microplate readers, flow cytometry, and fluorescence microscopy. AAT Bioquest provides spectral data to help you match the dye with appropriate filters and detectors, minimizing background and maximizing signal clarity.
Analytical Methods and Assay Integration
In ELISA and lateral flow assays, Fluorescein-labeled antibodies enable sensitive signal generation without radioactive isotopes. Conjugation chemistry typically involves amine-reactive isothiocyanate derivatives, which must be controlled for pH and temperature to preserve labeling efficiency. When integrating this dye into new workflows, verify compatibility with your buffer composition and detection strategy to avoid quenching or photobleaching artifacts.
Handling, Stabilization, and Troubleshooting
Proper handling is essential to retain the optical quality of Fluorescein (cas 2321075). Prepare fresh working solutions, protect from prolonged light exposure, and monitor pH, since fluorescence intensity can decline under acidic or strongly basic conditions. If you observe aggregation or reduced signal, check solvent choice, storage duration, and container type. Small lot-to-lot variations are minimized by sourcing from a quality-focused supplier such as AAT Bioquest.
Regulatory Considerations and Application Fit
For in vitro diagnostic development, confirm that Fluorescein meets relevant reagent-grade specifications and documentation standards. The consistent performance of this dye supports method validation and regulatory submissions when handled according to documented protocols. Use the specification table to align material attributes with your project requirements, and maintain batch records for traceability.
Best Practices and Key Takeaways
- Verify spectral properties against your detection system before scaling up
- Follow conjugation and quenching protocols to maximize labeling efficiency
- Document storage conditions and lot numbers for regulatory traceability
- Monitor pH and light exposure to maintain signal integrity
- Select AAT Bioquest grades that match diagnostic or research sensitivity requirements
FAQ
Reader questions
What is the recommended solvent for reconstituting Fluorescein (cas 2321075) from AAT Bioquest?
Use high-purity water or buffered aqueous solutions; slight alkalinity can improve solubility, but avoid prolonged exposure to extreme pH.
How does lot-to-lot consistency of Fluorescein affect assay reliability?
Tight lot-to-lot consistency minimizes variability in fluorescence intensity and labeling efficiency, supporting reproducible results across experiments and production batches.
Can Fluorescein conjugates be used in flow cytometry applications?
Yes, when protected from photobleaching and correctly filtered, Fluorescein conjugates provide bright, stable signals for multicolor flow cytometry panels.
What storage conditions maximize the shelf life of Fluorescein (cas 2321075)?
Store desiccated powder at 2–8°C in light-protected containers; minimize freeze-thaw cycles and avoid prolonged exposure to elevated temperatures.