cd61 integrin beta 3 antibody percpefluor 710 46061942 enables high-contrast imaging of beta 3 integrin expression in complex biological and clinical specimens. This near-infrared fluorescent probe supports quantitative flow cytometry, multiplex immunofluorescence, and intravital microscopy with minimal spectral overlap.
Designed for rigorous translational research, the conjugate bridges a well-characterized integrin antibody with a proprietary perfluorinated tracer for enhanced signal intensity and tissue penetration. The following sections detail target biology, assay performance, and operational guidance for advanced imaging applications.
| Product Identifier | Target | Excitation/Emission (nm) | Compatible Applications |
|---|---|---|---|
| 46061942 | Integrin Beta 3 (CD61) | 710 / ~730 | Flow cytometry, immunofluorescence, multiplex IHC, intravital imaging |
| Host Species | Mouse | Matched fluorophore | Optimized for human and murine samples |
| Isotype | IgG1 kappa | Purification method | Protein A/G affinity purification |
| Format | Lyophilized conjugate | Reconstitution | Recommended in DMSO or appropriate aqueous buffer per protocol |
Integrin Beta 3 Biology and Antibody Specificity
Integrin beta 3 (CD61) is a critical subunit forming the alphaIIbbeta3 and alphaVbeta3 receptors, which regulate platelet aggregation, endothelial cell migration, and tumor angiogenesis. The cd61 integrin beta 3 antibody percpefluor 710 46061942 selectively recognizes native and activated conformations of CD61, enabling precise spatial mapping in heterogeneous specimens.
By targeting conformational epitopes near the ligand-binding domain, this antibody minimizes cross-reactivity with related integrins. Such specificity is essential when analyzing complex tissues where integrin switching influences disease phenotypes and therapeutic response.
Multiplex Fluorescence Imaging Workflow
Implementing cd61 integrin beta 3 antibody percpefluor 710 46061942 in multiplex panels requires careful spectral planning to leverage the 710 nm excitation window. Pairing this probe with far-red or near-infrared dyes minimizes bleed-through, while optimized compensation strategies enhance quantitative accuracy across channels.
For fixed and permeabilized cells, standard fixation protocols preserve CD61 epitopes, whereas cryosectioning with mild detergents yields superior membrane integrity for intravital studies. Consistent sample preparation underpins reproducible signal intensity and accurate colocalization metrics.
Flow Cytometry and Quantitative Readouts
In suspension assays, cd61 integrin beta 3 antibody percpefluor 710 46061942 delivers low background and high signal-to-noise, facilitating robust population discrimination. Titration experiments and compensation controls are recommended to refine threshold settings and ensure linear dynamic range across patient cohorts.
The conjugation chemistry supports single-cell resolution in clinical specimens, allowing correlation of beta 3 expression with phosphorylation states and downstream signaling markers. Such multimodal flow cytometry accelerates translational insights into integrin-mediated pathways.
Protocol Optimization and Troubleshooting
Optimizing permeabilization duration, antibody concentration, and incubation temperature enhances staining uniformity in dense tissues. Empirical titration curves reveal saturation points, reducing non-specific labeling and conserving valuable reagent for high-content screening.
When background fluorescence appears, stringent post-stain washes with gentle detergents combined with appropriate secondary controls resolve non-specific binding. These steps safeguard data integrity in high-dimensional imaging experiments.
Translational Research and Clinical Assay Integration
Integrating cd61 integrin beta 3 antibody percpefluor 710 46061942 into standardized clinical assays supports reproducible quantification of beta 3 dynamics in oncology and vascular disease. Harmonized protocols across centers improve data comparability and facilitate meta-analyses of integrin-targeted interventions.
Ongoing validation studies define correlation thresholds between fluorescence intensity and protein surface density, informing cutoff definitions for diagnostic or predictive utility in patient stratification.
- Validate antibody specificity with isotype controls and knockout samples
- Optimize permeabilization and fixation to preserve CD61 epitopes
- Perform spectral unmixing when using multiple near-infrared labels
- Use serial dilutions to balance sensitivity, background, and reagent conservation
- Correlate imaging results with orthogonal methods such as Western blot or RNA expression
FAQ
Reader questions
Is this antibody suitable for both fixed and frozen tissue sections?
Yes, the conjugate performs well in both fixed and frozen sections when the appropriate permeabilization and blocking conditions are applied according to the validated assay protocol.
Can I use cd61 integrin beta 3 antibody percpefluor 710 46061942 in multiplex panels with other near-infrared dyes?
Absolutely; the 710 nm excitation and emission profile enables seamless incorporation into multiplex panels, provided spectral compensation is configured to account for any minor spillover into adjacent channels.
What sample types show the strongest CD61 labeling intensity?
Highly vascular tissues, endothelial cell monolayers, and invasive tumor margins typically exhibit robust labeling, correlating with elevated integrin beta 3 expression in angiogenic and migratory cell populations.
How should I determine the optimal antibody concentration for my specimen?
Perform a serial dilution titration across a representative subset of samples, then compare signal intensity and signal-to-noise ratios to identify the concentration that balances sensitivity with minimal background.