The Berthold Mithras LB 940 Microplate Reader is a precision instrument designed for high-throughput screening and detailed biochemical assays in modern laboratories. Engineered by Bionex Scientific, it combines advanced detection technology with user-friendly workflow integration to support research across drug discovery, molecular biology, and clinical diagnostics.
This system emphasizes sensitivity, flexibility, and data reliability, enabling scientists to focus more on experimental insights and less on instrument management. The following sections detail its performance features, assay compatibility, operational advantages, and common user inquiries.
| Model | Core Detection Modes | Read Speed | Dynamic Range |
|---|---|---|---|
| Berthold Mithras LB 940 | Absorbance, Fluorescence, Luminescence | <3 seconds per 96-well plate | |
| Integration Options | Microplate formats 6–384 well | Auto-discards and dual-read capabilities | Low-level assays with high signal fidelity |
| Software Suite | MithrasPro Data Evaluation | Protocol templates and export tools | Multimethod curve fitting and QC metrics |
| Footprint & Workflow | Compact bench design with tray loading | Batch and continuous run modes | Supports LIMS and automated lab environments |
Performance Specifications and Optical Design
Optical Path and Sensitivity
The Berthold Mithras LB 940 utilizes a monochromator-based optical system with selectable filters for absorbance and fluorescence endpoints as well as time-resolved luminescence. High-efficiency gratings and low-noise detectors deliver consistent signal quality, supporting sub-femtomole luminescence assays and linear absorbance readings from 0.1 to 3.0 optical density.
Microplate Handling and Throughput
Designed for medium-throughput environments, the LB 940 accommodates 6–384 well microplates with adjustable plate carriers and stackable packaging. Its gripper module enables rapid yet gentle transfer, reducing edge effects and carryover between runs while maintaining sample integrity.
Assay Compatibility and Application Scope
Biochemical and Cell-Based Assays
Scientists rely on this reader for enzyme-linked immunosorbent assays, cell viability, reporter gene, and GPCR signaling studies. The flexible detection modes allow side-by-side comparison of luminescent and fluorescent readouts within the same experimental workflow, improving data correlation.
ADME-Tox and Screening Metrics
In early-stage discovery, the LB 940 supports quantitative binding and inhibition assays with low Z′ factor variability. Researchers can track reaction kinetics using kinetic luminescence, enabling more precise EC50 and IC50 determination for lead optimization programs.
Workflow Integration and Laboratory Usability
Software Environment and Data Handling
MithrasPro software provides intuitive protocol setup, visual plate maps, and automated QC flags. Built-in wizards guide users through plate imports, background subtraction, and standard curve generation, while export options streamline reporting to GLP/GMP frameworks.
Physical Layout and Maintenance
The compact footprint allows seamless placement in standard laboratory benches, with minimal vibration interference from adjacent equipment. Modular components and sealed optics lower routine maintenance needs, and remote diagnostics reduce downtime for critical project timelines.
Operational Reliability and Compliance
Calibration, Validation, and Traceability
Each unit undergoes performance verification against reference standards, with traceable calibration certificates supporting 21 CFR Part 11 compliance when configured appropriately. Scheduled tasks within MithrasPro help laboratories document environmental monitoring and preventive maintenance activities.
Safety, Environment, and Power Management
Low-voltage operation and enclosed optical compartments minimize user exposure while maintaining consistent temperature stability. Energy-saving modes automatically adjust fan and lamp activity, aligning with institutional sustainability goals without compromising measurement accuracy.
Operational Advantages and Recommendations
- Optimize screening throughput with rapid read speeds and reliable plate handling
- Enhance data quality using built-in QC metrics and MithrasPro validation tools
- Future-proof your workflow with modular upgrades and software expansion options
- Ensure regulatory compliance with detailed audit trails and secure data management
FAQ
Reader questions
What types of assays can the Berthold Mithras LB 940 handle reliably?
The reader supports absorbance, fluorescence, and luminescence assays, including ELISA, cell proliferation, reporter gene, and ATP-based viability screens, with validated performance across 6–384 well microplates.
How does the optical system maintain signal consistency across a run?
Monochromator-based filtering and low-noise detectors reduce wavelength-dependent fluctuations, while automated plate positioning and background correction minimize edge effects and ensure uniform detection across the plate.
Can this reader integrate with automated liquid handling platforms and LIMS?
Yes, the LB 940 offers flexible communication modules and standardized data outputs that support integration with major liquid handlers and laboratory information systems, enabling seamless track-and-trrace workflows. Each system includes traceable calibration certificates, preventive maintenance schedules, and detailed installation qualification protocols, along with remote diagnostics and on-site service options to meet GLP and GMP requirements.